Studies on Crystallization of Peptidyl-prolyl cis-trans Isomerase and AreB from Aspergillus flavus
نویسندگان
چکیده
منابع مشابه
The characterization of a cyclophilin-type peptidyl prolyl cis-trans-isomerase from the endoplasmic-reticulum lumen.
A luminally located peptidyl prolyl cis-trans-isomerase (PPI) has been purified from bovine liver microsomes. It has a molecular mass of 20.6 kDa, and N-terminal sequencing demonstrates strong sequence similarity to the sequences of the cyclophilin B family. The enzyme catalyses the isomerization of the standard proline-containing peptide N-succinyl-Ala-Ala-Pro-Phe p-nitroanilide, as well as th...
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Peptidyl-prolyl cis-trans isomerases (EC 5.2.1.8) catalyse the interconversion of cis and trans peptide bonds and are therefore considered to be important for protein folding. They are also thought to participate in processes such as signalling, cell surface recognition, chaperoning and heat-shock response. Here we report the soluble expression of recombinant Mycobacterium tuberculosis peptidyl...
متن کاملThe gene for cyclophilin (peptidyl-prolyl cis-trans isomerase) from Schizosaccharomyces pombe.
Cyclophilin (CPH) is an ubiquitous 17-kD cytosolic protein with high binding affinity for the immunosuppressant cyclosporin A (1). CPH is identical to peptidyl-prolyl cis-trans isomerase, an enzyme catalyzing the isomerisation of proline imidic peptide bonds in oligopeptides and accelerating the rate-limiting steps in the refolding of certain proteins in vitro (2, 3). We have used degenerate an...
متن کاملCell cycle-dependent phosphorylation of nucleophosmin and its potential regulation by peptidyl-prolyl cis/trans isomerase.
Nucleophosmin (NPM) is a ubiquitously expressed phosphoprotein involved in many cellular processes. Phosphorylation is considered the major regulatory mechanism of the NPM protein, associated with diverse cellular events. In this study, we characterized the phosphorylation status of several physiological phosphorylation sites of NPM, especially the newly confirmed in vivo site threonine 95 (Thr...
متن کاملNovel spiroannulated 3-benzofuranones. Synthesis and inhibition of the human peptidyl prolyl cis/trans isomerase Pin1.
The novel 3H-spiro[1-benzofuran-2-cyclopentan]-3-one skeleton has been made accessible by different routes. One- and two-step protocols lead to tricyclic and tetracyclic benzofuranones 2 and 3, respectively. A four-step synthesis to spirocompound 4 is described. The novel spirocyclic benzofuranones display modest to no inhibition of the human peptidyl prolyl cis/trans isomerase Pin1.
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ژورنال
عنوان ژورنال: Journal of Bacteriology & Mycology: Open Access
سال: 2017
ISSN: 2469-2786
DOI: 10.15406/jbmoa.2017.05.00121